The cycling event was a fundraiser for Project Hero, a national nonprofit that provides services for injured veterans and first responders.
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The motorcyclist was transported to University Medical Center with a head injury after a collision with a Regional Transportation Commission bus in downtown Las Vegas.
A Toyota caught fire after being rear-ended by a Mustang and both of its occupants died at the scene, police said. A 24-year-old man was arrested on DUI-related charges.